Tips & Tricks for Those Working with Equine Breeding – Sperm VitalStain

23 Mar 2026 | News, Tips & Tricks

When working with insemination or fertility assessment in stallions, it is crucial to quickly determine what proportion of the sperm cells are actually alive. Motility does not tell the whole story – even immotile sperm can be alive. This is where Sperm VitalStain comes in as one of the most reliable tools in the laboratory.

Why choose Nidacon’s Sperm VitalStain? • Easy to use – ready-to-use one-step solution • Clear distinction between live and dead sperm: o Dead cells are stained red by eosin o Live cells remain light/transparent against the dark background of nigrosin

• Provides reliable vitality percentages, especially important when motility is reduced or variable
• Suitable for fresh, chilled, and frozen stallion semen – where vitality is critical for fertility assessment
• Designed for breeding stations aiming to improve the quality of their analyses

Scientific reference
Amorim O.M. et al, Can a simple and cheap semen assessment help with fertility? Journal of Equine Veterinary Science 145 (2025)

In short: With Sperm VitalStain, you can make better decisions regarding dosage and dilution – and ultimately improve pregnancy outcomes.

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