Maximize Stallion Semen Survival with the Triple Crown Protocol

Aug 2025 | Tips & Tricks

Maximize Stallion Semen Survival with the Triple Crown Protocol

– Three steps to boost post-thaw viability and reproductive success

Give your breeding program the winning edge with this proven protocol designed to preserve sperm quality through every stage of processing and cryopreservation.

Image

1. Dilution

Start by diluting the semen sample with Botusemen Gold – a high-performance extender formulated to protect sperm viability during handling and preparation.

2. Gradient Separation

Enhance sample quality using EquiPure and a gradient-based separation technique. This step isolates motile, morphologically normal spermatozoa, optimizing the fertility potential of your sample.

3. Cryopreservation

Freeze the sample using Botucrio, a cryoprotective agent specifically engineered to preserve sperm integrity throughout the freezing and thawing process.

By following this three-step approach, you significantly improve post-thaw motility and viability, increasing the likelihood of successful artificial insemination outcomes.

Triple Crown – because every dose deserves the best chance at success.

Other Tips & Tricks

Maximize Sperm Recovery with Red Cushion

Maximize Sperm Recovery with Red Cushion – For gentle, efficient semen centrifugation in equine reproduction Streamline your workflow and protect sperm quality with Red Cushion – a smart solution that improves recovery rates while minimizing cellular damage. Cushioned...

How stable are Nidacon products?

Nidacon Colloidal products, EquiPure and BoviPure, have a shelf-life of two years and un-opened they can be stored at room temperature. However, once opened, it becomes important to preserve their stability by storing them in the refrigerator. The same shelf life...

How to best store BotuSemen Gold?

Ensure proper storage conditions for optimal quality of BotuSemen Gold. Avoid high temperatures exceeding 35°C. For the safest storage; place BotuSemen Gold in the refrigerator. When preparing the solution, allow the powder to fully dissolve in water (approximately 30...

Dont waste any product!

Sometimes, not all the solution is used.  Did you know that the BotuSemen extenders (all types) can be frozen after resuspension. An alternative is to safely store them refrigerated for up to three days. BotuCrio can be re-frozen once if the whole solution is not...