EquiPure – 100 ml
Increasing the quality and viability of equine sperm.
EquiPure is a density gradient product designed to increase the quality and viability of equine sperm by separating the sperm by means of density centrifugation prior to insemination, freezing, IVF, or semen sexing. EquiPure not only eliminates a high proportion of the abnormal spermatozoa but also removes the source of reactive oxygen species, most bacteria, and many viruses (EAV). This significantly increases sperm survival and their fertilizing potential. Nidacon’s EquiPure is used by breeders, animal reproduction scientists, and veterinarians all over the world.
Features
Improves fertility rates, especially in sub-fertile stallions
Improves freezability of equine sperm
Removes bacteria and other unwanted components from raw ejaculates or inseminating doses.
Removes Equine Arteritis Virus (EAV) particles
Studies performed on the hygienic aspects of semen storage for AI demonstrate that the addition of antibiotics to extenders is often not enough to eliminate the detrimental influence of bacteria on sperm motility, viability and fertility. EquiPure centrifugation prior to freezing removes bacteria and many viruses (EAV).
Components
Silane-coated silica, Potassium chloride, Sodium chloride, Purified water, HEPES, EDTA, Glucose, Calcium lactate, Sodium pyruvate
Storage and Stability
Store unopened bottles at 2 to 40ºC and avoid temperatures above or below these values. Under these conditions EquiPure has a shelf-life of 24 months. The expiry date is shown on both bottles and cartons. Open and close bottles under aseptic conditions. After opening,
store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
Precautions and warnings
• When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination
• If available, use sealed buckets during centrifugation to avoid creation of aerosols
• Clean accidental spills using a dampened cloth or paper. EquiPure causes floors and benches to be extremely slippery
• EquiPure does not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer
• Do not use any solution which shows evidence of bacterial contamination or if stopper accidently comes in contact with unsterile surfaces
• Do not use contents if tamper-evident seal is broken
• Does not contain antibiotics, use aseptic procedures
General recommendations
If the sperm to be treated are already frozen, the thawed ejaculate needs to equilibrate for approximately 15 minutes in BotuSemen before centrifugation. This is necessary to allow the sperm to rehydrate and return to their correct density.
Documents
References
Effect of Centrifugal Fractionation Protocols on Quality and Recovery Rate of Equine Sperm.
A.J Edmont, S.P Brinsko, DD Varner et al.
Theriogenology [0093- 691X] Edmond, (2012) vol. 77 no 5 pp 959.
How to improve the freezability and fertility of stallion semen using silanated silica-particle solution (EquiPure)
Juan C. Samper
AAEP-meeting 2010.
Semen Processing for the Subfertile Stallion.
Varner D.D. et al
Journal of Veterinary Science, (2008) Vol 28, no 11, pp 677-685.
Use of a Silane-Coated Particle Solution to Enhance the Quality of Ejaculated Semen in Stallions.
Varner D.D. et al
Theriogenology, (2002) Volume 58, Issue 2, pp 317-320.
Does the Microbial Flora in the Ejaculate Affect the Freezability of Stallion Sperm?
Ortega-Ferrusola C. et al
Reprod. Dom Anim 44, (2009) pp 518-522.
Evaluation of Cryopreserved-thawed Stallion Sperm Before and after Density Gradient Centrifugation with Silane-coated Silica Particles (EquiPure)
Stoll A, et al
Theriogenology 70, (2008) pp. 576-591.
Insemination Doses: How Low Can We Go?
Brinsko S.P. et al
Theriogenology 66, (2006) 543-550.
Advanced Methods for Handling and Preparation of Stallion Semen.
Loomis P.R. et al
Anim Reprod Sci., (2006) Feb;91(3-4):237-47.
Effect of Density-Gradient Centrifugation on Quality and Recovery Rate of Equine Spermatozoa.
Edmont A.J., Varner D.D. et al
Anim Reprod Sci. Issues 3-4, (2008) September; page 318.
Effective Removal of Equine Arteritis Virus From Stallion Semen Geraghty
R.M. et al
Equine Veterinary Journal, Volume 38, Number 3, (2006) pp. 224-229(6).
Application of EquiPure to improve stallion sperm quality and its equine arteritis virus decontamination.
Golnik, W et al
Medycyna Weterynaryjna,(2004), Vol 60; Part 5, pages 508-511.
FAQ
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