EquiPure – 100 mL
Increasing the quality and viability of equine sperm.
EquiPure is a density gradient product designed to increase the quality and viability of equine sperm by separating the sperm by means of density centrifugation prior to insemination, freezing, IVF, or semen sexing. EquiPure not only eliminates a high proportion of the abnormal spermatozoa but also removes the source of reactive oxygen species, most bacteria, and many viruses (EAV). This significantly increases sperm survival and their fertilizing potential. Nidacon’s EquiPure is used by breeders, animal reproduction scientists, and veterinarians all over the world.
Features
Improves fertility rates, especially in sub-fertile stallions
Improves freezability of equine sperm
Removes bacteria and other unwanted components from raw ejaculates or inseminating doses.
Removes Equine Arteritis Virus (EAV) particles
Studies performed on the hygienic aspects of semen storage for AI demonstrate that the addition of antibiotics to extenders is often not enough to eliminate the detrimental influence of bacteria on sperm motility, viability and fertility. EquiPure centrifugation prior to freezing removes bacteria and many viruses (EAV).
Components
Silane-coated silica, Potassium chloride, Sodium chloride, Purified water, HEPES, EDTA, Glucose, Calcium lactate, Sodium pyruvate
Storage and Stability
Store unopened bottles at 2 to 40ºC and avoid temperatures above or below these values. Under these conditions EquiPure has a shelf-life of 24 months. The expiry date is shown on both bottles and cartons. Open and close bottles under aseptic conditions. After opening,
store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
Precautions and warnings
• When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination
• If available, use sealed buckets during centrifugation to avoid creation of aerosols
• Clean accidental spills using a dampened cloth or paper. EquiPure causes floors and benches to be extremely slippery
• EquiPure does not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer
• Do not use any solution which shows evidence of bacterial contamination or if stopper accidently comes in contact with unsterile surfaces
• Do not use contents if tamper-evident seal is broken
• Does not contain antibiotics, use aseptic procedures
General recommendations
If the sperm to be treated are already frozen, the thawed ejaculate needs to equilibrate for approximately 15 minutes in BotuSemen before centrifugation. This is necessary to allow the sperm to rehydrate and return to their correct density.
Documents
References
Comparison of Nanoparticles and Single-Layer Centrifugation for Separation of Dead from Live Stallion Spermatozoa
Bisiau C et al, Vet Sciences, 2027-07, Vol 11, p.307
The effect of sex-sorting on stallion semen quality
Samper JC et al, Journal of Equine Veterinary Science 125 (2023) 104
Successful use of sexed stallion semen for in vitro embryo production; a preliminary study
Pouya Dini et al (Samper), Journal of Equine Veterinary Science 125 (2023) 71
Fertility of conventional and of sex-sorted stallion sperm using Sexed ULTRA TM Genesis III technology
Samper JC et al, Journal of Equine Veterinary Science 125 (2023) 103
Age-related DNA damage in stallions: an ongoing investigation
Griffin RA et al, Journal of Equine Veterinary Science 125 (2023) 16
Successful use of sex-sorted frozen semen in a commercial OPU-ICSI program
Ducheyne KD et al, Journal of Equine Veterinary Science 125 (2023) 73
Equine Semen Cryopresrvation
Sanches R et al
Equine Assisted Reproduction, ISBN 978-958-59883-1-6, Section 3, 2019
Semen Processing for the Subfertile Stallion
Varner D.D et al
Journal of Veterinary Science, Vol 28, no 11 (2008)
Increasing Stallion Fertility
Quarterback news, December 15, 2013. pp. 78-80
Use of a Single-layer Density Centrifugation Method Enhances Sperm Quality in Cryopreserved-Thawed Equine Spermatozoa
Stoll, A et al. Journal of Equine Vet Science, 2012
Insemination Doses: How Low Can We Go?
Brinsko S P et al
Theriogenology 66 (2006) 543-550
Dept of Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 78843-4475, USA
Total RNA isolation from stallion sperm and testis biopsies
Pranab j Das et al, Texas A&M
Theriogenology 74 (2010) 1099-1106
Does the Microbial Flora in the Ejaculate Affect the Freezeability of Stallion Sperm?
C Ortega-Ferrusola et al
Reprod. Dom Anim 44, (2009) pp 518-522
Use of a Silane-Coated Particle Solution to Enhance the Quality of Ejaculated Semen in Stallions
Varner D.D et al
Theriogenology, Volume 58, Issue 2, Pages 317-320
Pregnancy rates in mares inseminated with 0.5 or 1 million sperm using hysteroscopic or transrectally guided deep-horn insemination techniques
S.S Hayden, DD Varner et al.
Theriogenology 2012.
How to improve the freezeability and fertility of stallion semen using a silanated silica-particle solution (EquiPure™)
Samper, JC 2010
Stoll A, et al.
Theriogenology 70 (2008) pp. 576-591.
Advanced Methods for Handling and Preparation of Stallion Semen
Loomis et al
Anim Reprod Sci. 2006 Feb;91(3-4):237-47
Effective Removal of Equine Arteritis Virus From Stallion Semen
Geraghty, R.M.
Equine Veterinary Journal, Volume 38, Number 3, May 2006 , pp. 224-229(6)
Effect of Centrifugal Fractionation Protocols on Quality and Recovery Rate of Equine Sperm
A.J Edmont, S.P Brinsko, DD Varner et al.
Theriogenology [0093-691X] Edmond year:2012 vol.:77 no:5 p:959
Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa
Edmond A.J, Varner D.D et al.
Abstracts, Animal rep Science 2008
Simple and economic colloidal centrifugation protocols may be incorporated to the clinical equine sperm processing procedure.
Gutierrez-Cepeda et al. Animal Mediciene and Surgery Dept, Vet Faculty, Madrid, Spain.
Effect of single layer colloidal centrifugation prior to equine semen cryopreservation on sperm
chromatin integrity.
Gutiérrez-Cepeda, L.*a; Fernández, A.a; Crespo, F.b; Ramirez, M.A.c; Serres, C.a
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, UCM,
Ciudad Universitaria, 28040, Madrid.
The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders of the fragmentation dynamics of thawed equine sperm DNA
Gutierrez-Cepeda et al.
Acta Veterinaria Scandinavica 2012, 54:72
EquiPure™ Improves Fertility of Age Sub-Fertile Stallions after Artificial Insemination (AI) with Cooled semen
Khalifa TAA et al
Abstract, presented at ESDAR conference Eger, Hungary 2010
Effect of non-sperm cells removal with single-layer colloidal centrifugation on myeloperoxidase concentration in post-thaw equine semen
Ponthier, J, et al.
Theriogenology 80 (2013) 1082-1087
Effect of density gradient centrifugation with three different media on stallion semen quality
Sabatini, C et al.
Journal of Equine Vet Science 34 (2014) 77
Recovery rate and sperm quality after centrifugation of stallion sperm in different gradient concentrations
Love, CC et al
Journal of Equine Vet Science 34 (2014) 63
Total RNA isolation from stallion semen sperm and testis biopsies
Das J Pranab, Varner DD et al.
Theriogenology 74 (2010) 1099-1106
Density gradient centrifugation of sperm from a subfertile stallion and effect of seminal plasma addition on fertility (Redigrad)
Gaetano M et al
Anim Rep Science 126(2011) 96-100
”In conclusion separation of frozen-thawed equine sperm via centrifugation through an EquiPure® density gradient increased total an progressive motility, normal morphology, mitochondrial membrane potential, and plasma membrane alteration of the separated sperm”
Resumen: Más allá de las técnicas tradicionales de procesamiento y evaluación seminal: Centrifugación coloidal e integridad de la cromatina espermática en lacriopreservación de semen equino. Resultados preliminares.
Gutiérrez-Cepeda, L.*a; Fernández, A.a; Crespo, F.b; Ramirez, M.A.c; Serres, C.a
Aumento de la fertilidad mediante técnicas de procesamiento avanzado de semen: Caso clínico
Ayuso Hernando, M.; Gutierrez-Cepeda, L.; Villalva Orero, M.; Serres Dalmau, C.
Servicio de Reproducción, Hospital Clínico Veterinario Complutense, UCM
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