BoviPure 100 mL
A product used for isolation and purification of bovine spermatozoa.
BoviPure is designed to be diluted with BoviDilute to desired concentrations in order to form either a one-layer- or a two-layer gradient for density centrifugation. The pellet retrieved after centrifugation contains only normal, purified spermatozoa.
Features
Colloidal silica particles coated with silane, in an isotonic salt solution.
Unopened bottles have a shelf-life of 2 years.
pH of 7.4 – 8.0.
Terminally sterilised by autoclaving.
Bottles are made of borosilicate glass and silicone stoppers with tamper-evident seals.
Bottles and stoppers have been M.E.A. tested.
Components
Silane-Coated Silica, Calcium Chloride, Potassium Chloride, Sodium Chloride, purified water, HEPES, EDTA, Glucose
Storage and Stability
Store unopened bottles at between 2 – 40°C. Avoid temperatures above or below these values. When stored at these temperatures, BoviPure or BoviDilute have a shelf life of 2 years. The expiry date is shown on the labels.
Open and close bottles under aseptic conditions. After opening, store the bottles at between 2 – 8°C.
No antibiotics, unstable additives or preservatives have been added to BoviPure.
Precautions and warnings
When retrieving the sperm pellets, follow the instructions given in the product insert in order to avoid inadvertent re-contamination.
Use sealed buckets or centrifuge tubes during centrifugation to avoid the creation of aerosols.
Clean accidental spills with dampened cloth or paper. BoviPure can cause floors and bench tops to be slippery.
BoviPure is non-flammable and does not present a fire or combustion hazard. A material safety data sheet is available upon request.
General recommendations
To ensure best possible results, use aseptic conditions at all times.
Documents
References
Pre-freezing selection of Holstein bull semen with the BoviPure colloid as double- or single-layer centrifugation improves the post-thawing quality
Salman A et al, Animal Reproduction Science 2023-11, Vol 258, article 107344
Selection of reference genes for normalizing gene expression data across seasons in spermatozoa of water buffalo (Bubalus bubalis)
Vasisth et al, International Journal of Biometeorology (2024) 68:1397–1409
IMPROVED CRYOPRESERVATION OF IN VITRO-PRODUCED BOVINE EMBRYOS
USING A CHEMICALLY DEFINED FREEZING MEDIUM
Buff, S et al Theriogenology, 2012
In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival and establishemnt of pregnancy.
Moreno D et al
Theriogenology 84(2015) 1053-1060
A comparison of BoviPure and Percoll on bull sperm separation protocols for IVF.
Samardzija M, et al, Anim Reprod Sci. 2006 Feb;91(3-4):237-47
Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
“Although sperm evaluation parameters did not differ between our two protocols we observed that cleavage rates and embryo production appeared to be superior following BoviPure® compared to Percoll® separation.”
Effects of bovine spermatozoa preparation on embryonic development in vitro.
Samardzija M et al
Reprod Biol Endocrinol. 2006 Nov 13;4 (1):58 17101040
“So, we concluded that BoviPure® could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos”.
Retained Functional Integrity of Bull Spermatozoa after Double Freezing and Thawing Using PureSperm® Density Gradient Centrifugation
WMC Maxwell 1 , I Parrilla 2 , I Caballero 2 , E Garcia 2 , J Roca 2 , EA Martinez 2 , JM Vazquez 2 and D Rath 3
Reproduction in Domestic Animals, 2007 Oct, Volume 42 Issue 5, Pages 489 – 494
“These results demonstrate that bull semen can be frozen and thawed, followed by a second freeze-thawing cycle of a population of spermatozoa selected by PureSperm®, with retained motility and functional integrity. This points to the possibility of using double frozen spermatozoa in bovine artificial insemination programmes and to the potential benefits of PureSperm® density gradient centrifugation for the application of cryopreserved bull spermatozoa to other biotechnological procedures such as flow cytometric sex sorting followed by re-freezing and thawing.”
DEVELOPMENT OF pROCEDURES FOR SEX-SORTING FROZEN-THAWED BOVINE SPERMATOZOA
SL Underwood, R Bathgate et al.
Reproduction in Domestic Animals, 2008 doi: 10.1111/j.1439-0531.2008.01127.x
“Sperm were prepared for sorting by density gradient separation in either PureSperm((R)) or BoviPuretrade mark”
EFFECT OF DENSITY GRADIENT CENTRIFUGATION WITH TRYPSIN ON THE IN VIVO FERTILIZING CAPABILITY OF BOVINE SPERM
- A. Blevins, M. de la Rey and N. M. Loskutoff
Reproduction, Fertility and Development, 2008;20(7):784-8
“In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.
BOVINE SPERM SEPARATION BY SWIM-UP AND DENSITY GRADIENTS (PERCOLL AND BOVIPURE): EFFECT ON SPERM QUALITY, FUNCTION AND GENE EXPRESSION
M E Arias et al
Reproductive biology 17 (2017) 126-132
“Additionally, BoviPure density gradient is a good alternative for sperm separation, since although motility is lower than with Percoll, important sperm quality parameters, such as DNA integrity, DCM, plasma and acrosomal membrane integrity and gene expression, are not affected”
EFFECT OF DENSITY GRADIENT CENTRIFUGATION WITH TRYPSIN ON THE FERTILIZING CAPABILITY OF BOVINE SPERM
Blevins et al
Reproduction, Fertility and Development 20(1) 84-84, 2007
“BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods.”
Other species using BoviPure
Canine:
BoviPure® density-gradient centrifugation procedure enhances the quality of fresh and cryopreserved dog epididymal spermatozoa
Galarza D.A et al, Animal Reproduction Science 242 (2022) 107003
Porcine:
Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm
Transcriptome by RNA-Seq
Lian, Yu et al, Front. Vet. Sci., 19 July 2021 Sec. Animal Reproduction – Theriogenology Volume 8 – 2021
A technical assessment of the porcine ejaculated spermatozoa for a sperm-specific RNA-seq analysis
Godia M et al, SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE2018, 2018, VOL. 64, NO. 4, 291–303
Ram:
Effect of microfluidic and density gradient techniques to select frozen-thawed epididymal sperm from Bighorn rams
Arroyo E et al, Theriogenology Wild, 2024, Vol 5 p.100108
Goat:
Intracytoplasmic sperm injection (ICSI) of prepubertal goat oocytes using fresh and frozen-thawed semen
Menendez-Blanco et al, Small Ruminant Research, Volume 170, January 2019, Pages 137-142
Guinea Pig:
Acquisition of fertilization competence in guinea pig spermatozoa under different capacitation protocols
Canon-Beltran K et al, Theriogenology, 2023-03, Vol.198, p.231-240
Deer:
Selection of red deer spermatozoa with different cryo resistance using density gradients
Garcia-Alvarez et al, Reprod Dom Anim 2016; 51: 895–900
FAQ
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