BotuSemen Special 100 mL
A Semen Extender based on skimmed milk with the addition of cholesterol.
Cholesterol maintains sperm plasma membrane stability during the cooling process.. The addition of this essential lipid component makes BotuSemen Special ideal for pre-freezing centrifugation. BotuSemen Special is suitable for all stallion, especially for those with spermatozoa that are sensitive to cooled transport.
How to Prepare:
Carefully open the sachet, taking care not to touch the inside of the packet with your fingers, and empty the contents of the sachet into the bottle of sterile water. Replace lid on bottle, and shake well. Wait 15 minutes prior to using. This allows the cholesterol to distribute properly for optimal results. Warm to 37°C before extending semen. Be sure extender remains at the same temperature as the semen to prevent cold shock of the semen.
Usage:
Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen.
Use dilutions of 30 to 50 million/ml for cooled transport. Extend 1:1 (v/v and wait 15 minutes for an insemination with non-cooled semen. In case of samples requiring centrifugation before cooled transport, resuspend the sperm at a rate of between 30 to 50 million/ml
Features
Contains Penicillin K 1 g/l and Gentamicine 1 g/l.
Components
Composition: Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, cholesterol and excipients.
Storage and Stability
Shelf-life: Two years in powder form.
After mixing, the extender can be stored between 2-8°C for up to 3 days.
One year frozen after re-constitution in water.
Precautions and warnings
N/A
General recommendations
BotuSemen Special is highly recommended for prefreezing centrifugation and is suitable for spermatozoa with sensitivity to cooled transport.
Documents
Safety Data Sheet – View PDF
Product insert (latest version) – n/a
References
Techniques for Semen Processing in Equine Breeding Management and Artificial Insemination, Second Edition.
Samper J.C.
Saunders Elsevier (2009)
Comparison between two extenders to cool equine semen for 24 hours before freezing.
Melo, C. et al.
Vet. e Zootec (2008). v.15, n.2,p.325-331.
FAQ
N/A