BotuSemen Gold 100 ml
A casein-based Semen Extender with added cholesterol.
BotuSemen Gold is based on casein which in high concentrations contributes to a better protection during cooling and transportation, improving pregnancy rates. Milk casein phosphoproteins protect sperm cells from the action of seminal plasma proteins (HSP-1, HSP-2) and Ca++ ions, prolonging spermatic longevity and facilitating semen transport. Numerous research papers report that seminal samples diluted in casein present a higher rate of binding to the oocyte, improving pregnancy rates.
BotuSemen GOLD is suitable for all stallions, especially for those with need of better protection during cooling and transportation. BotuSemen Gold also provides a liquid translucent which makes visualization for cushion centrifugation easier.
How to Prepare:
Carefully open the sachet, taking care not to touch the inside of the packet with your fingers, and empty the contents of the sachet into the bottle of sterile water. Replace lid on bottle, and shake well. Wait 30 minutes prior to using. This allows the casein to distribute properly for optimal results. Warm to 37°C before extending semen. Be sure extender remains at the same temperature as the semen to prevent cold shock of the semen.
Usage:
A temperature of between 5°C or 15°C should be used for cooled semen transport as well as for pre-freezing centrifugation.
Use dilutions of 50 million/ml for cooled transport, adhering to the minimum of 2 parts of extender to 1 part of semen.
In case of samples requiring centrifugation before cooled transport, resuspend the spermatozoa at a rate of at least 100×106 sptz/mL. DO NOT exceed the appropriate concentrations of cholesterol.
It is important to adhere to the dilutions in the table below:
Spermatic Concentration Dilution (v:v)
< 90×106/mL 1:1 Centrifuged Resuspended 100×106sptz/mL
90-120×106/mL 2:1
130-160×106/mL 3:1
170-210×106/mL 4:1
220-250×106/mL 5:1
260-300×106/mL 6:1
Features
Contains Penicillin K 1 g/l and Gentamicine 1 g/l.
Components
Composition: Casein, sugars, amino acids, antioxidants, cholesterol and excipients.
Storage and Stability
Shelf-life: Two years in powder form.
After mixing, the extender can be stored between 2-8°C for up to 3 days.
Can be stored one year frozen after re-constitution in water.
Precautions and warnings
N/A
General recommendations
BotuSemen GOLD is suitable for all stallions, especially for those with suspected premature sperm capacitation.
Documents
Safety Data Sheet – View PDF
Product insert (latest version) – n/a
References
Techniques for Semen Processing in Equine Breeding Management and Artificial Insemination, Second Edition.
Samper J.C.
Saunders Elsevier (2009)
Comparison between two extenders to cool equine semen for 24 hours before freezing.
Melo, C. et al.
Vet. e Zootec (2008). v.15, n.2,p.325-331.
FAQ
N/A