BotuSemen 100 ml (Water included)
A Semen Extender for transporting or preserving cooled semen.
The result of years of research, BotuSemen is a Semen Extender based on skimmed milk for transporting cooled semen or preserving it between 5°C and 15°C, as well as to dilute the ejaculate for pre-freezing centrifugation. Suitable for most stallions, and provides the best cost/benefit ratio. BotuSemen’s current formulation enables the sperm safe transportation until the moment of insemination.
How to Prepare:
Add all the content of this packet in 100 mL of sterile water and shake very well.
Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen.
Use dilutions of 30 to 50 million/ml for cooled transport. Extend 1:1 (v/v and wait 15 minutes for an insemination with non-cooled semen. In case of samples requiring centrifugation before cooled transport, resuspend the sperm at a rate of between 30 to 50 million/ml.
Contains Penicillin K 1 g/l and Gentamicine 1 g/l.
Composition: Skimmed milk powder UHT sterilized, sugars, amino acids, antioxidants, and excipients.
Storage and Stability
Shelf-life: Two years in powder form.
After mixing, the extender can be stored between 2-8°C for up to 3 days.
Can be stored one year frozen after re-constitution in water.
Precautions and warnings
Suitable for most stallions.
Techniques for Semen Processing in Equine Breeding Management and Artificial Insemination, Second Edition.
Saunders Elsevier (2009)
Comparison between two extenders to cool equine semen for 24 hours before freezing.
Melo, C. et al.
Vet. e Zootec (2008). v.15, n.2,p.325-331.