BotuCrio 25 ml

Cat. No. BTC-025 Category:

A cryoprotectant medium for equine sperm.

BotuCrio, Nidacon’s special cryoprotectant medium for equine sperm, employs a novel and innovative formulation, developed over many years of research and fertility trials at the State University of São Paulo, Brazil, as well as during many multicenter field tests. The, BotuCrio, combines the perfect combination of component substances in the perfect proportions to best protect cells from damage caused by freezing, and to protect the fertilizing ability of sperm after thawing.



Combining EquiPure and BotuCrio, enables the freezing of semen from almost any stallion, particularly stallions whose semen could not previously be frozen commercially with useable sperm yields.

BotuCrio is shipped only to Europe, frozen and extra handling cost will be added. Minimum order size 10 pcs.

* Sold in Europe only


Better Motility Parameters: BotuCrio contains a lower concentration of glycerol and, when combined with one of several amides, results in better sperm integrity, sperm viability, better post-thaw total and progressive motility, and higher sperm velocity than other commercially available, freezing extenders.

Better Fertility Rates: Studies have indicated that stallion sperm frozen in BotuCrio have better fertility than provided by other extenders. This is crucial since better-than-average in-vitro results during field experiments have been shown to provide higher pregnancy rates after insemination.

Fewer Inseminations per Pregnancy: The higher pregnancy rates achieved with semen frozen in BotuCrio results in a higher pregnancy rate per cycle and fewer inseminations per pregnancy, translating to lower costs when breeding a mare.

Gentamicine 0.5 g/l


Egg yolk, Carbo hydrates, Amino acids, Preservatives, Gentamicin (0.5 g/l), Excipients, Cryo protectants, Ultra Pure Water

Storage and Stability

Shelf life: 1 year frozen. 3 days at 2-8°C  after thaw.

Precautions and warnings


General recommendations


How to improve the freezability and fertility of stallion semen using silanated silica-particle solution (EquiPure)

Juan C. Samper
AAEP-meeting 2010.

86 Effects of three cryopreservation systems on longevity of stallion sperm after thawing.

Alvarenga M.A. et al
Reproduction, Fertility and Development (2008) 20(1) 123-124.

Post-thaw characteristics and fertility of stallion semen frozen in extenders with different cryoprotectants.

Samper J.C. et al
Animal Reproduction Science (2008)

Freezing of stallion epididymal sperm.

Papa F.O. et al
Animal Reproduction Science 107 (2008) 293-301.

Effects of concentration of seminal plasma on cryopreservation of equine spermatozoa.

Rocha A.S. et al
Acta Scientiae Veterinariae. 38 (2010) (Supl 2); s690.

Cryopreservation of equine spermatozoa comparing different freezing rates combined with commercial extenders: laboratorial analysis.

Terraciano P.B. et al
Ciência Rural, Santa Maria (2008), v.38, n.7, pp.1972-1977.

Comparison between two extenders to cool equine semen for 24 hours before freezing.

Melo C.M. et al
Vet. E Zootec. V.15, (2008) n.2, p.325-331.

Transport of equine ovaries for assisted reproduction.

B.I Ribeiro et al
Animal Reproduction Sci (2008); 108(1-2): 171-179.